Egyptian Henbane learn more about names for this taxon Hyoscyamus muticus Trusted Image of Hyoscyamus muticus; Map of Hyoscyamus muticus. Expert(s). Expert: Notes: Reference for: Other Source(s). Source: Germplasm Resources Information Network (GRIN), – , database (version ). Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
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The effects of colchicine on the growth of plantlets were assessed one month after treatment. It is sometimes hyocsyamus in countries such as Egypt, Pakistan and India for its medicinal alkaloids, which may be exported to countries such as Germany. Wet and dry weight was increased by tetraploid induction Table 1as expected.
The wild plants are used in traditional local medicine. The meristem tip of plantlets in their leaf stage were first covered with cotton Fig. This study was carried out with the financial assistance of the Institute of Biotechnology and the Deputy Research of Ferdowsi University in Mashhad-Iran.
Flow cytometry analysis of ploidy levels: Since, key facts with regards to the physiology of this plant are unknown, however, basic physiological studies are still required to understand this stringent process in Hyoscyamus muticus. This study was conducted for two yearsin the agricultural research glasshouse at Ferdowsi University of Mashhad-Iran. The plant survival was not affected hyoscyqmus the concentration and time of exposure of colchicines.
Tetraploid Induction of Hyoscyamus muticus L. The epidermic cells of tetraploids as shown in Fig. Identification of tetraploid regenerants from cotyledons of diploid watermelon cultured in vitro. The ploidy level of treated plants near their flowering stage, was measured by flow cytometry Partec PA, Germany. Histogram comparison of average number of chloroplast between diploid and tetraploid cells.
Hyoscyamus muticus – Wikipedia
The initiation of growth occurred about 10 days after treatment, whereas untreated plantlets continue to grow without any delay. After about 40 days of growth, all colchicines treated plantlets had significantly shorter shoots than untreated plantlets.
In vitro induction of tetraploidy in mulberry Morus alba L. For this purpose, three plantlets were chosen from both the diploid control and tetraploid groups. It can be efficiently used for ploidy determination in plants growing in fields or in the greenhouse Dolezel et al.
Since, the plant meristem consists of many cells, as well as obtaining tetraploids, it is possible to obtain mixoploids chimeras consisting of diploid and tetraploid tissue after colchicine treatment Koutoulis et al. The leaves may also be applied as a poultice to relieve pain. Effects of double haploid production in wheat. In this study, the peak that represents the G 1 phase of the tetraploid cell cycle was located on channelwhereas for control this peak was on channel Morphological characteristics such as size and thickness of leaves, flowers, seeds, epidermis and guard cells, as well as the number of chloroplast in guard cells were considered as other parameters for identifying putative tetraploid plants.
Although, the seed is not the ultimate economic product in this species, it is still required for propagation. Endopolyploidy in diploid and tetraploid maize Zea mays L. The most widely applied and best studied chemical that induces polyploids is colchicine, mutiucs alkaloid extracted from seeds or corms of the autumn crocus Colchicum autumnae or Colemid, its synthetic equivalent Hassawi and Liang, ; Poehlman, Retrieved from ” https: Colchicine induced polyploidy in Abies.
Pakistan Journal of Biological Sciences, The biology and biochemistry of underground organs. Male sterility was a visible effect of tetraploid induction of H. Colchicine disrupts mitosis by binding to tubulin, inhibiting the formation of microtubules and the polar migration of chromosomes, resulting in cells with doubled chromosome number Panda et al. Presence of an inhibitor agent could be another possible explanation for high proportion of nuclei in G 2 phase of cell cycle in these species.
The stomata frequency and sizes of diploid a, c and tetraploid b, d Egyptian henbane Hyoscyamus muticus. In vitro induction of banana autotetraploids by colchicine treatment of micropropagated diploids.
The overall conclusion is that the method used is reliable for routine in vivo production of polyploids in Egyptian henbane. Wikimedia Commons has media related to Hyoscyamus muticus. In addition we also report reliable morphological markers to identify putative tetraploids in this species. In vitro tetraploid induction and generation of tetraploids frome mixoploids in hop Humulus lupulus L.